The PCR is widely used in nucleic acids-based research and is increasingly applied in clinical molecular diagnostics. Closed-tube (homogeneous) methods have a number of advantages for practical ...
What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...
Real-time PCR or quantitative PCR (Polymerase Chain Reaction) is an advancement of the standard PCR technique, which enables continuous monitoring of the reaction and collection of data in real time.