Investigation of peak purity failure during HPLC method validation led to discovery of a co-eluting impurity under the main peak. Spectral analysis, including three-dimensional modelling, was used to ...
When conducting reproducible and interference-free analysis by liquid chromatography, the purity of the solvents used, particularly the purity of water, is crucial for the sensitivity of this method, ...
High-performance liquid chromatography (HPLC) or ultra-high-performance liquid chromatography (UHPLC) serves as the front end of the analysis, reducing sample complexity before mass detection.
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