pH is a critical parameter in method development and routine enzyme assay measurement. At the ideal pH value, called optimal pH, enzymes have their highest activity. Accurate and reliable ...

pH is a measure of how acidic or alkaline a solution is. The diagram below shows what happens to an enzyme when denaturation occurs. Each enzyme has an optimum pH but it also has a working range of pH ...

This article describes a simple method to determine the optimum pH for the activity of one enzyme in a coupled enzyme assay. Decoupling of the two-step reaction is achieved by heat denaturing the ...

The factors that affect enzyme catalysis (that is, enzyme activity) will definitely affect the rate of enzymatic reactions, but the factors that affect the rate of enzymatic reactions do not ...

Acid phosphatase hydrolyses phosphate ester bonds under an optimum pH of 4.8 and an optimum temperature of 37 °C. In this experiment you will use an artificial substrate, para-nitrophenylphosphate ...